PDB entry 1b27

View 1b27 on RCSB PDB site
Description: structural response to mutation at a protein-protein interface
Class: hydrolase/hydrolase inhibitor
Keywords: RNAse-inhibitor complex, hydrolase/hydrolase inhibitor complex
Deposited on 1998-12-04, released 1998-12-09
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.195
AEROSPACI score: 0.4 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d1b27a_
  • Chain 'B':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d1b27b_
  • Chain 'C':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d1b27c_
  • Chain 'D':
    Compound: protein (barstar)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11540 (0-89)
      • see remark 999 (0)
      • engineered (25)
      • engineered (67)
    • Uniprot P11540 (0-89)
      • see remark 999 (0)
      • engineered (25)
      • engineered (67)
    • Uniprot P11540 (0-89)
      • see remark 999 (0)
      • engineered (25)
      • engineered (67)
    Domains in SCOPe 2.07: d1b27d_
  • Chain 'E':
    Compound: protein (barstar)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11540 (Start-89)
      • engineered (25)
      • engineered (67)
    • Uniprot P11540 (Start-89)
      • engineered (25)
      • engineered (67)
    • Uniprot P11540 (Start-89)
      • engineered (25)
      • engineered (67)
    Domains in SCOPe 2.07: d1b27e_
  • Chain 'F':
    Compound: protein (barstar)
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11540 (Start-89)
      • engineered (25)
      • engineered (67)
    • Uniprot P11540 (Start-89)
      • engineered (25)
      • engineered (67)
    • Uniprot P11540 (Start-89)
      • engineered (25)
      • engineered (67)
    Domains in SCOPe 2.07: d1b27f_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download)
    >1b27A (A:)
    aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
    gklpgksgrtwreadinytsgfrnsdrilyssdwliykttdhyqtftkir
    

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download)
    >1b27B (B:)
    aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
    gklpgksgrtwreadinytsgfrnsdrilyssdwliykttdhyqtftkir
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >1b27C (C:)
    aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
    gklpgksgrtwreadinytsgfrnsdrilyssdwliykttdhyqtftkir
    

    Sequence, based on observed residues (ATOM records): (download)
    >1b27C (C:)
    vintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnregk
    lpgksgrtwreadinytsgfrnsdrilyssdwliykttdhyqtftkir
    

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download)
    >1b27D (D:)
    mkkavingeqirsisdlhqtlkkelalpeyygenldalwdaltgwveyplvlewrqfeqs
    kqltengaesvlqvfreakaegaditiils
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >1b27E (E:)
    mkkavingeqirsisdlhqtlkkelalpeyygenldalwdaltgwveyplvlewrqfeqs
    kqltengaesvlqvfreakaegaditiils
    

    Sequence, based on observed residues (ATOM records): (download)
    >1b27E (E:)
    kkavingeqirsisdlhqtlkkelalpeyygenldalwdaltgwveyplvlewrqfgaes
    vlqvfreakaegaditiils
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >1b27F (F:)
    mkkavingeqirsisdlhqtlkkelalpeyygenldalwdaltgwveyplvlewrqfeqs
    kqltengaesvlqvfreakaegaditiils
    

    Sequence, based on observed residues (ATOM records): (download)
    >1b27F (F:)
    kkavingeqirsisdlhqtlkkelalpeyygenldalwdaltgwveyplvlewrqfeqsk
    qltengaesvlqvfreakaegaditiils