PDB entry 1b21

View 1b21 on RCSB PDB site
Description: deletion of a buried salt bridge in barnase
Class: hydrolase
Keywords: microbial ribonuclease, alpha/beta protein
Deposited on 1998-12-03, released 1998-12-09
The last revision prior to the SCOP 1.75 freeze date was dated 1999-12-22, with a file datestamp of 2007-07-20.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.188
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    Domains in SCOP 1.75: d1b21a_
  • Chain 'B':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    Domains in SCOP 1.75: d1b21b_
  • Chain 'C':
    Compound: protein (barnase)
    Species: Bacillus amyloliquefaciens
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    • Uniprot P00648 (Start-109)
      • engineered (68)
      • engineered (92)
    Domains in SCOP 1.75: d1b21c_
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1b21A (A:)
aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
gklpgksgstwreadinytsgfrnsdrilyssnwliykttdhyqtftkir

    Sequence, based on observed residues (ATOM records): (download) 
    >1b21A (A:)
qvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnreg
klpgksgstwreadinytsgfrnsdrilyssnwliykttdhyqtftkir

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1b21B (B:)
aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
gklpgksgstwreadinytsgfrnsdrilyssnwliykttdhyqtftkir

    Sequence, based on observed residues (ATOM records): (download) 
    >1b21B (B:)
vintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnregk
lpgksgstwreadinytsgfrnsdrilyssnwliykttdhyqtftkir

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >1b21C (C:)
aqvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnre
gklpgksgstwreadinytsgfrnsdrilyssnwliykttdhyqtftkir

    Sequence, based on observed residues (ATOM records): (download) 
    >1b21C (C:)
qvintfdgvadylqtyhklpdnyitkseaqalgwvaskgnladvapgksiggdifsnreg
klpgksgstwreadinytsgfrnsdrilyssnwliykttdhyqtftkir